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Methanolic extracts of coca tea baggage were being analyzed by GC/MS in the total scan method. Alkaloids were being identified by comparison of electron influence spectra with Those people of reliable requirements.
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Alkaloids were being determined by comparison of entire scan electron affect spectra with that of reference criteria. Quantitation was achieved by functioning the MSD in the chosen ion monitoring manner (SIM). The following ions were being monitored for each compound: nicotine, m/z
Coca tea bags were randomly picked from each source, weighed, and afterwards tea ready because of the addition of one bag to 180 ml of deionized water at 94°C. The tea bag was preserved in the new water for three min, removed and also the pH of the infusion determined.
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Extracts had been purified by solid-phase extraction (SPE) accompanied by Examination by gasoline chromatography/mass spectrometry (GC/MS). Coca tea prepared from Peruvian and Bolivian coca tea baggage was also analyzed by SPE-GC/MS assay. Additionally, urine specimens were being analyzed from a person who consumed a single cup of Peruvian coca tea and just one cup of Bolivian coca tea on independent situations. Urine samples had been analyzed by immunoassay (TDxR) and SPE-GC/MS. Investigation of coca tea baggage and coca tea indicated that copyright, benzoylecgonine, ecgonine methyl ester and trans
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